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| Registros recuperados: 19 | |
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| Vicari,Marcelo Ricardo; Noleto,Rafael Bueno; Artoni,Roberto Ferreira; Moreira-Filho,Orlando; Bertollo,Luiz Antonio Carlos. |
| Karyotype data are presented for distinct species of the genus Astyanax from four rivers belonging to three different hydrographic basins of the State of Paraná, Brazil (Verde River - Tibagi basin, Açungui River - Ribeira basin, and Santo Antônio and Jaguariaíva Rivers - Jaguariaíva basin). Three karyotypic forms were identified, here denominated karyotype A (2n = 50 chromosomes, with 8m+18sm+10st+14a, and thirteen 18S rDNA sites); karyotype B (2n = 50 chromosomes, with 8m+18sm+10st+1f4a, and four 18S rDNA sites); and karyotype C (2n = 48 chromosomes, with 10m+16sm+10st+12a, and eight 18S rDNA sites). The pattern of constitutive heterochromatin was similar among the three karyotypic forms, with few differences. The 5S rDNA corresponds to a synapomorphic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype evolution; Cytotaxonomy; RDNA sites; Biogeography. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200002 |
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| Santos,Dayane Petik dos; Felicetti,Denise; Baumgärtner,Lucas; Margarido,Vladimir Pavan; Blanco,Daniel Rodrigues; Moreira-Filho,Orlando; Lui,Roberto Laridondo. |
| ABSTRACT Auchenipteridae is divided into subfamilies Centromochlinae and Auchenipterinae. Parauchenipterus is included in the latter and is subject of taxonomic discussions concerning its validation or synonymization with Trachelyopterus. Herein, three species from two hydrographic basins were cytogenetically analyzed: Parauchenipterus striatulus from Doce River and two sympatric species, P. galeatus and Trachelyopterus coriaceus, from the Araguaia River. Diploid number of 58 chromosomes was verified for all species, but P. striatulus has different karyotype formula from the others. The three species have heterochromatin located in terminal regions of almost all chromosomes and in pericentromeric region on acrocentric chromosomes. Simple NORs was verified... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryptic species; FISH; Parauchenipterus; Problematic taxonomy; 5S rDNA. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100214 |
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| Marques,Marilza Barbosa de Almeida; Moreira-Filho,Orlando; Garcia,Caroline; Margarido,Vladimir Pavan. |
| Two Siluriformes species endemic to the São Francisco River basin were characterized by conventional and differential cytogenetic analyses involving C-banding, Ag-nucleolar organizer region (NOR) and chromomycin A3 (CMA3) staining, and FISH (fluorescent in situ hybridization) with 18S and 5S rDNA probes. Conorhynchus conirostris presents a higher diploid number (2n = 60) than those detected in Pimelodidae representatives, whereas Lophiosilurus alexandri, with a karyotype of 2n = 54 chromosomes, presents a chromosomal constitution similar to that found in the family Pseudopimelodidae. Plesiomorphic characteristics such as single NORs at terminal positions are found in both species, as revealed by CMA3 and silver nitrate staining, and FISH with a 18S rDNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Incertae sedis; Pimelodidae; Pseudopimelodidae; RDNA; Siluriformes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200008 |
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| Vicari,Marcelo Ricardo; Almeida,Mara Cristina de; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Artoni,Roberto Ferreira. |
| We used differential staining techniques (BSG, GTG, AgNO3, DAPI and CMA3 banding) and fluorescent in situ hybridization (FISH) with 5S and 18S probes to investigated the karyotypic and cytogenetic chracteristics of Prochilodus lineatus specimens from a population in Vila Velha state park (Parque Estadual de Vila Velha, Ponta Grossa, Paraná state, southern Brazil). The specimens studied showed the same karyotype as that found in other P. lineatus populations, i.e. 2n = 54 biarmed chromosomes (40m + 14 sm) and c-positive heterochromatin preferentially located pericentromerically in all chromosomes. The presence of partial or totally heterochromatic supernumerary chromosomes with numeric intra-individual variation was confirmed in the analyzed population. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish cytogenetics; Prochilodus; Karyotype; Minor and major rDNA; NOR polymorphism. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400008 |
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| Santos,Emanoel Oliveira dos; Deon,Geize Aparecida; Almeida,Rafael Bonfim de; Oliveira,Ezequiel Aguiar de; Nogaroto,Viviane; Silva,Hugmar Pains da; Pavanelli,Carla Simone; Cestari,Marta Margarete; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando; Vicari,Marcelo Ricardo. |
| Abstract Parodontidae is a small group of fish and some species are particularly difficult to identify due to the lack of sufficiently consistent morphological traits. Cytogenetically, the species possess 2n = 54 chromosomes and are either sex-homomorphic or sex-heteromorphic (regarding its chromosomes). We evaluated data on color, tooth morphology, cytogenetics, and mitochondrial markers (COI) in Apareiodon specimens from the Aripuanã River (Amazon basin) and the results were compared to other congeneric taxa. Morphological results show an overlap of body color and tooth morphology to other known Apareiodon. The cytogenetics data showed that the 2n = 54 chromosomes, 50 m/sm + 4 st and, a ZZ/ZW sex chromosome system in Apareiodon sp. are common to other... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosomal differentiation; Hidden diversity; Repetitive DNA; Sex chromosomes. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000300365 |
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| Kantek,Daniel L. Z.; Peres,Wellington A. M.; Buckup,Paulo A.; Moreira-Filho,Orlando. |
| Specimens of Imparfinis schubarti (Gomes, 1956) collected in the Piumhi river drainage, state of Minas Gerais, Brazil, were studied cytogenetically. The river was diverted from the Rio Grande Basin into the São Francisco basin in the early 1960s. All individuals presented 2n = 58 chromosomes, including 18 metacentric, 34 submetacentric and six subtelocentric chromosomes. A secondary constriction was observed in the interstitial region of the long arm of the largest chromosome pair, coinciding with the NOR. A single conspicuous heterochromatic block located in the largest pair of metacentric chromosomes was observed, adjacent to the secondary constriction. A detectable 18S rDNA probe hybridization region occurs in only one chromosome pair and is synthenic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Transposed river; Subclade nemuroglanis; FISH; São Francisco River Basin. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-46702009000400018 |
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| Pucci,Marcela Baer; Nogaroto,Viviane; Moreira-Filho,Orlando; Vicari,Marcelo Ricardo. |
| Abstract Eukaryotic genomes consist of several repetitive DNAs, including dispersed DNA sequences that move between chromosome sites, tandem repeats of DNA sequences, and multigene families. In this study, repeated sequences isolated from the genome of Characidium gomesi were analyzed and mapped to chromosomes in Characidium zebra and specimens from two populations of C. gomesi. The sequences were transposable elements (TEs) named retroelement of Xiphophorus (Rex); multigene families of U2 small nuclear RNA (U2 snRNA); and histones H1, H3, and H4. Sequence analyses revealed that U2 snRNA contains a major portion corresponding to the Tx1-type non-LTR retrotransposon Keno, the preferential insertion sites of which are U2 snRNA sequences. All histone... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mobile DNA; Histones; Karyotype evolution; SnRNA; WZ/ZZ. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400585 |
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| Bellafronte,Elisangela; Mariguela,Tatiane Casagrande; Pereira,Luiz Henrique Garcia; Oliveira,Claudio; Moreira-Filho,Orlando. |
| In the past years, DNA barcoding has emerged as a quick, accurate and efficient tool to identify species. Considering the difficulty in identifying some Parodontidae species from the La Plata basin and the absence of molecular data for the group, we aimed to test the effectiveness of DNA barcoding and discuss the importance of using different approaches to solve taxonomic problems. Eight species were analyzed with partial sequences of Cytochrome c oxidase I. The mean intraspecific K2P genetic distance was 0.04% compared to 4.2% for mean interspecific K2P genetic distance. The analyses of distance showed two pairs of species with K2P genetic divergence lower than 2%, but enough to separate these species. Apareiodon sp. and A. ibitiensis, considered as the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apareiodon; COI gene; Cytogenetics; Fish identification; Morphology. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000300497 |
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| Castro,Jonathan P.; Moura,Maurício O.; Moreira-Filho,Orlando; Shibatta,Oscar A.; Santos,Mateus H.; Nogaroto,Viviane; Vicari,Marcelo R.; Almeida,Mara C. de; Artoni,Roberto F.. |
| Two populations of the Astyanax scabripinniscomplex, isolated by a waterfall with over 100 meters depth and inhabiting different altitudes of the same river (1850 m a.s.l. and 662 m a.s.l.) were compared in reproductive data, geometric morphometry, tooth morphology, anal-fin rays counts, and karyotype, in order to test the hypothesis of speciation between the two populations. The results in the geometric morphometry analysis showed differences between the populations. Discriminant function analysis (DFA) and canonical variance analysis revealed sexual dimorphism. Secondary sexual characters, such as hooks in the anal fin rays of the males are absent in the lower altitude population. Both populations had the same macro karyotype structure, except for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Allopatric populations; Cytogenetics; Evolution; Geometric morphometry; Paraíba do Sul River. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252014000200429 |
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| Oliveira,Maria Lígia M. de; Utsunomia,Ricardo; Pansonato-Alves,José Carlos; Scacchetti,Priscilla C.; Primo,Cleberson C.; Vicari,Marcelo R.; Artoni,Roberto F.; Centofante,Liano; Moreira-Filho,Orlando; Oliveira,Claudio; Foresti,Fausto. |
| Trichomycterus is a specious fish genus within Trichomycterinae and displays remarkable karyotype diversity. However, knowledge about their genomic structure and location of repetitive sequence is still limited. In order to better understand the karyotype diversification, we analyzed nine species of Trichomycterus using classical and molecular cytogenetic techniques. Results revealed a conserved diploid chromosome number of 2n=54 chromosomes in all analyzed species, although remarkable differences on the constitutive heterochromatin distribution were observed. In addition, while the 18S rDNA showed a conserved distribution pattern, the 5S rDNA sites showed a quite diverse location considering the analyzed species. Remarkably, both ribosomal genes were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Karyotype diversity; Repetitive DNA; RDNA; Supernumerary chromosomes. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252016000200211 |
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| Kavalco,Karine Frehner; Pazza,Rubens; Bertollo,Luiz Antonio Carlos; Moreira-Filho,Orlando. |
| Cytogenetic data about satellite DNA distribution in four Astyanax species (Characidae) from the Paraitinga river, Paraíba do Sul river basin, Brazil, are presented. In order to characterize the constitutive heterochromatin, C-banding, chromomycin A3 and DAPI fluorescence staining, as well as fluorescence in situ hybridization (FISH) with the satellite DNA As-51 probe were performed. A. scabripinnis and A. parahybae presented 2n = 50 and 2n = 48 chromosomes, respectively. The heterochromatin was located in the pericentromeric and terminal regions of many chromosomes, corresponding to GC-positive regions and to the As-51 satellite DNA in terminal regions. A. intermedius and A. giton, both with 2n = 50 chromosomes, showed little heterochromatin, mostly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astyanax; Fluorescence in situ hybridization (FISH); Heterochromatin; Karyotypic diversity; Satellite DNA. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000400005 |
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| Registros recuperados: 19 | |
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